Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg
نویسندگان
چکیده
Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all infected tissues, being older roots those which displayed the most intense GUS staining. To our knowledge, this is the first report of Pentalinon andrieuxii susceptibility to Agrobacterium tumefaciens-mediated genetic transformation.
منابع مشابه
Providing a mathematical model for measuring the expression of GUS gene was transferred temporarily through xylem vessels using RT-PCR and probe Gold nanoparticles
Gene transfer to plants and the production of transgenic plants with various purposes, such as improving the performance and quality, resistance to pests, diseases, etc., and of great importance are carried out Gene transfer to plants performs to evaluate the transient and permanent gene expression. Transient expression is quick, easy and simple and is not influenced by position effect compare ...
متن کاملProviding a mathematical model for measuring the expression of GUS gene was transferred temporarily through xylem vessels using RT-PCR and probe Gold nanoparticles
Gene transfer to plants and the production of transgenic plants with various purposes, such as improving the performance and quality, resistance to pests, diseases, etc., and of great importance are carried out Gene transfer to plants performs to evaluate the transient and permanent gene expression. Transient expression is quick, easy and simple and is not influenced by position effect compare ...
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